Department of Plant Biology, 166 Plant Biology Building, East Lansing, MI 48824-1312,Phone (517) 355-4683, Fax(517)353-1926
Barbara B. Sears;Professor of Plant Biology
Publications since 2000, in reverse chronological order:
GuhaMajumdar, M and BB Sears. 2004. Chloroplast DNA base substitutions: an experimental assessment. Mol. Gen. Genomics, in press.
GuhaMajumdar, M, S Baldwin, and BB Sears. 2004. Chloroplast mutations induced by 9-aminoacridine hydrochloride are independent of the plastome mutator in Oenothera. Theor. Appl Genet 108 (3):543 – 549
Riekhof, WR, ME Ruckle, TA Lydic, BB Sears, C Benning. 2003. The sufolipids 2'O-acyl-sulfoquinovosyldiacylglycerol and sulfoquinovosyldiacylglycerol are absent from a Chlamydomonas reinhardtii mutant deleted in SQD1. Plant Physiol 133:864-874
Hupfer, H., M. Swiatek, S. Hornung, R. G. Herrmann, R. M. Maier, W.-L. Chiu, and B.B. Sears. 2000. Complete nucleotide sequence of the Oenothera elata plastid chromosome, representing plastome I of the five distinguishable Euoenothera plastomes. Mol Gen Genet 263:581-585.
Other Publications in peer-reviewed journals in chronological order:
Wurtz, E.A., B.B. Sears, D.K. Rabert, H.S. Shepherd, N.W. Gillham and J.E. Boynton. 1979. A specific increase in chloroplast gene mutations following growth of Chlamydomonas in 5-fluorodeoxyuridine. Mol. Gen. Genet. 170:235-242.
Sears, B.B., J.E. Boynton and N.W. Gillham. 1980. The effect of gametogenesis regimes on the chloroplast genetic system of Chlamydomonas reinhardtii. Genetics 96:95-114.
Sears, B.B. 1980. Disappearance of the heteroplasmic state for chloroplast markers in zygospores of Chlamydomonas reinhardtii. Plasmid 3:18-34.
Sears, B.B. 1980. Changes in chloroplast genome composition and recombination during the maturation of zygospores of Chlamydomonas reinhardtii. Current Genetics 2:1-8.
Birky, C.W., Jr., K.P. VanWinkle-Swift, B.B. Sears, J.E. Boynton and N.W. Gillham. 1981. Frequency distributions for chloroplast genes in Chlamydomonas zygote clones: Evidence for random drift. Plasmid 6:173-192.
Alt, J., P. Westhoff, B.B. Sears, N. Nelson, E. Hurt, G. Hauska and R.G. Herrmann. 1983. Genes and transcripts for the polypeptides of the cytochrome f/b6 complex from spinach and thylakoid membranes. EMBO Journal 2:979-986.
Sears, B.B. and R.G. Herrmann. 1985. Plastome mutation is a post-transcriptional defect. Current Genetics 9:521-528.
Chiu, W.-L. and B.B. Sears. 1985. Recombination between chloroplast DNAs does not occur in sexual crosses of the biparental plant Oenothera. Mol. Gen. Genet. 198:525-528.
Moroney, J., N.E. Tolbert, and B.B. Sears. 1986. Complementation analysis of the inorganic carbon concentrating mechanism of Chlamydomonas reinhardtii. Mol. Gen. Genet. 204:199-203.
Epp, M.D., J.L. Erion, B.B. Sears, and W. Stubbe. 1987. The plastome mutator of Oenothera continues to function as originally described. Mol. Gen. Genet. 206:515-518.
Chiu, W.-L., W. Stubbe, and B.B. Sears. 1988. Plastid inheritance in Oenothera: organelle genome modifies the extent of biparental plastid transmission. Current Genetics 13:181-189.
Blasko, K., S.A. Kaplan, K.G. Higgins, R. Wolfson, and B.B. Sears. 1988. Variation in copy number of a 24-base pair tandem repeat in the chloroplast DNA of Oenothera hookeri strain Johansen. Current Genetics 14:287-292.
Wolfson, R. and B.B. Sears. 1989. A reduction in the abundance of calcium oxalate crystals in Oenothera improves the yield of intact chloroplasts. Plant, Cell and Environment 12:109-112.
Sears, B.B. and K.L. Klomparens. 1989. Leaf tip cultures of the evening primrose allow stable, aseptic culture of mycoplasma-like organisms. Can. J. Plant Path. 11:343-348.
Sears, B.B., P.-O. Lim, N. Holland, B.C. Kirkpatrick, K.L. Klomparens. 1989. Isolation and characterization of DNA from a mycoplasmalike organism. Mol. Plant-Microbe Interactions 2:175-180.
Lim, P.O. and B.B. Sears. 1989. 16S rRNA sequence shows that plant-pathogenic "mycoplasmalike organisms" are evolutionarily distinct from animal mycoplasmas. J. Bacteriol. 171:5901-5906.
Stine, M., B.B. Sears, and D. Keathley. 1989. Inheritance of plastids in hybrids of white spruce and blue spruce. Theor. Appl. Genet. 78:768-774.
Chiu, W.-L., E.M. Johnson, S.A. Kaplan, M. Sokalski, K. Blasko, R. Wolfson, and B.B. Sears. 1990. Oenothera chloroplast DNA polymorphisms associated with plastome mutator activity. Mol. Gen. Genet. 221:59-64.
Johnson, E.M. and B.B. Sears. 1990. Membrane composition and physiological activity of plastids from an Oenothera plastome mutator-induced chloroplast mutant. Plant Physiol. 92:254-261.
Johnson, E.M. and B.B. Sears. 1990. Structure and expression of cytochrome f in an Oenothera plastome mutant. Current Genetics 17:529-534.
Khairallah, M.M., M.W. Adams and B.B. Sears. 1990. Mitochondrial DNA polymorphisms of Malawian bean lines:Further evidence for two major gene pools. Thr.App.Genet. 80:753-761.
Johnson, E.M., L.S. Schnabelrauch and B.B. Sears. 1991. A plastome mutation affects processing of both chloroplast and nuclear DNA-encoded plastid proteins. Mol. Gen. Genet. 225:106-112.
Lim, P.-O and B.B. Sears. 1991. Genome size of plant-pathogenic mycoplasmalike organism resembles that of animal-pathogenic mycoplasmas. J. Bacteriol. 173:2128-2130.
Wolfson, R., K.G. Higgins, and B.B. Sears. 1991. Evidence for replication slippage in the evolution of Oenothera chloroplast DNA. Molecular Biology and Evolution 8:709-720.
Khairallah, M.M., M.W. Adams, and B.B. Sears. 1991. Mitochondrial genome size variations and restriction fragment length polymorphisms in three Phaseolus species. Theor. Appl. Genet. 82:321-328.
Sears, B.B. and M.B. Sokalski. 1991. The Oenothera plastome mutator: effect of UV-irradiation and nitroso-methyl urea on mutation frequencies. Mol. Gen. Genet. 229:245-252.
Lim, P.-O. and B.B. Sears. 1991. DNA sequence of the ribosomal protein genes rpl2 and rps19 from a plant-pathogenic mycoplasmalike organism. FEMS Microbiology Letters 84:71-74.
Lim, P.-O., B.B. Sears, and K.L. Klomparens. 1992. Membrane properties of a plant-pathogenic mycoplasmalike organism. J. Bacteriol. 174(3):682-686.
Lim, P.-O. and B.B. Sears. 1992. Evolutionary relationships of a plant-pathogenic mycoplasmalike organism and Acholeplasma laidlawii deduced from two ribosomal protein gene sequences. J. Bacteriol. 174(8):2606-2611.
Chiu, W.-L. and B.B. Sears. 1992. Electron microscopic localization of replication origins in Oenothera chloroplast DNA. Mol. Gen. Genet. 232:33-39.
Khairallah, M.M., B.B. Sears, and M.W. Adams. 1992. Mitochondrial restriction fragment length polymorphisms in wild Phaseolus vulgaris L.: Insights on the domestication of the common bean. Theor. Appl. Genet. 84:915-922.
Glick, R. and B.B. Sears. 1993. Large unidentified open reading frame in plastid DNA (ORF2280) is expressed in chloroplasts. Plant Mol. Biol. 21:99-108.
Chiu, W.-L. and B.B. Sears. 1993. Plastome-genome interactions affect plastid transmission in Oenothera. Genetics. 133:989-997.
Toth, K.F., N. Harrison and B.B. Sears. 1994. Phylogenetic relationships among members of the class Mollicutes deduced from rps3 gene sequences. Int. J. Syst. Bacteriol. 44:119-124.
Glick, R. and B.B. Sears. 1994. Genetically-programmed chloroplast dedifferentiation as a consequence of plastome-genome incompatibility in Oenothera. Plant Physiol. 106:367-373.
Sears, B.B. and K.P. VanWinkle-Swift. 1994. The salvage-turnover-repair (STOR) model for uniparental inheritance in Chlamydomonas: DNA as a source of sustenance. J. Heredity. 85:366-376.
Seemüller, E., B. Schneider, R. Mäuer, U. Ahrens, X. Daire, H. Kison, K.-H. Lorenz, G. Firrao, L. Avinent, B.B. Sears and E. Stackenbrandt. 1994. Phylogenetic classification of plant-pathogenic mollicutes by sequence analysis of 16S rRNA. Int. J. Syst. Bacteriol. 44:440-446
Padovan, A.C., K.S. Gibb, A. Bertaccini, M. Vibio, R.E. Bonfiglioli, P.A. Magarey, and B.B. Sears. 1995. Molecular detection of the Australian grapevine yellows phytoplasma and comparison with grapevine yellows phytoplasmas from Italy. Aust. Jour. Grape and Wine Res. 1(1):25-31.
Sears B.B., L.L. Stoike, W.L. Chiu. 1996. Proliferation of direct repeats near the Oenothera chloroplast DNA origin of replication. Mol. Biol. Evol. 13:850-863.
Chang T.L., L.L. Stoike, D. Zarka, G. Schewe, B.B. Sears. 1996. Characterization of primary lesions caused by the plastome mutator of Oenothera. Current Genetics 30: 522-530.
Sears, B.B., J.I. Wood, G. Schewe, K.L. Klomparens. 1997. Effect of carbon dioxide and oxygen on phytoplasma abundance in Oenothera leaftip cultures. Physiological and Molecular Plant Pathology 50:255-287.
Stoike, L. and B.B. Sears. 1998. Plastome mutator induced alterations arise in Oenothera chloroplast DNA through template slippage. Genetics 149:347-353.
Review Articles in chronological order:
Sears, B.B. 1980. Review: Elimination of plastids during spermatogenesis and fertilization in the plant kingdom. Plasmid 4:233-255.
Herrmann, R.G., P. Seyer, R. Schedel, K. Gordon, C. Bisanz, P. Winter, J.W. Hildebrandt, M. Wlaschek, J. Alt, A.J. Driesel and B.B. Sears. 1980. The plastid chromosome of several dicotyledons, pp. 97-112. In Biological Chemistry of Organelle Formation (Th. Bucher, W. Sebald, H. Weiss, eds.). Springer-Verlag, Berlin.
Herrmann, R.G., P. Westhoff, J. Alt, P. Winter, J. Tittgen, C. Bisanz, B.B. Sears, N. Nelson, E. Hurt, G. Hauska, A. Viebrock and W. Sebald. 1982. Identification and characterization of genes of the thylakoid membrane. In Molecular Biology of Plants (O. Cifferi, ed.). Academic Press. pp. 143-154.
Sears, B.B. 1983. Genetics and evolution of the chloroplast. In Stadler Symposium, vol. 15:119-139.
Börner, T. and B.B. Sears. 1986. Plastome mutants. Plant Molecular Biology Reporter 4:69-92.
Khairallah, M.M., M.W. Adams and B.B. Sears 1990. Mesoamerican and Andean gene pools of beans: Evidence from mitochondrial RFLPs of bean lines from Malawi. Ann. Rep. Bean Imp. Coop. 33:84-85.
Khairallah, M.M., B.B. Sears, and M.W. Adams. 1991. Mitochondrial restriction fragment length polymorphisms in wild Phaseolus vulgaris L. Ann. Rep. Bean Imp. Coop. 34:139-140.
Khairallah, M.M., B.B. Sears, and M.W. Adams. 1991. Estimation of the mitochondrial genome size of three Phaseolus species. Ann. Rep. Bean Imp. Coop. 34:141-142.
Sears, B.B. and B.C. Kirkpatrick. 1994. Unveiling the evolutionary relationships of plant-pathogenic mycoplasmalike organisms. ASM News, 60(6):307-312.
Kirkpatrick, B.C., N.A. Harrison, I-M Lee, H. Neimark, B.B. Sears. 1995. Isolation of mycoplasma-like organism DNA from plant and insect hosts. Chap. B2, pgs. 105-117, In: Molecular and Diagnostic Procedures in Mycoplasmology, vol. 1 (S. Razin and J. Tully, eds.), Academic Press, Inc., San Deigo.
Sears, B.B. 1998. Replication, recombination, and repair in the chloroplast genetic system of Chlamydomonas. In: The Molecular Biology of Chloroplasts and Mitochondria of Chlamydomonas:. Kluwer Academic Publ. Advances in Photosynthesis Series vol. 7 (J.D. Rochaix, M. Goldschmidt-Clermont and S. Merchant, Eds.), chapter 7, pg 115-138.
